Fossil Fuel Bioprocessing projects

Geological and Geochemical Assessment of Biosouring Mitigation Strategies at the Cardinal Field Using Core Samples, etc.

Our pilot biosouring experimentation using the silicate GeoBioCell micromodel has been successfully completed using a soil enrichment culture, as well pure culture isolates of the sulfate-reducing bacterium Desulfovibrio vulgaris. In addition, core samples of oil-bearing subsurface sandstone reservoirs from the BP Cardinal field have been used to successfully construct a real reservoir rock GeoBioCell. The real rock GeoBioCell has been successfully inoculated with the enrichment and isolate cultures. Fluorescent tracers have identified flow paths and microbial metabolisms within individual rock pores. We are now closely coordinating GeoBioCell experiments with the UC Berkeley-LBNL large-scale biosouring column experiments.

project Highlights



Published in 2014

Influence of Mg2+ on CaCO3 Precipitation During Subsurface Reactive Transport Tested in a Micromodel Pore Network, V. Boyd, Y. Hongkyu, C. Zhang, M. Oostrom, N. Hess, B. W. Fouke, A. J. Valocchi, C. J. Werth, Geochimica et Cosmochimica Acta, 135, pp. 321-335, 2014. 


Halomonas sulfidaeris-Dominated Microbial Community Inhabits 1.8 km-deep Subsurface Cambrian Sandstone Reservoir, Y. V. Dong, C. G. Kumar, N. Chia, P. J. Kim, P. Miller, N. D. Price, I. O. Cann, T. M. Flynn, R. A. Sanford, I. G. Krapac, R. A. Locke, P. Y. Hong, H. Tamaki, W. T. Liu, A. G. Hernandez, C. L. Wright, M. A. Mikel, J. L. Walker, M. Sivaguru, G. Fried, A. Yannearell, R. I. Mackie, and B. W. Fouke, Environmental Microbiology, 15(12), pp. 1-14, doi: 10.1111/1462-2920.12325, 2014. 


Temperature and Sulfate Availability Structure Microbial Communities in Four Alaskan North Slope Hydrocarbon Reservoirs, Y. Piceno, E. Dubinsky, F. Reid, L. Tom, P. Hu, M. Conrad, M., M. Bill, C. Hubbard, C. Graff, J. Han, W. Stringfellow, J. Hanlon, T. Hazen, G. Andersen, B. W. Fouke, Frontiers in Microbiology, V. 5, Article 511, pp. 1-16. Doi:10.3389/fmicb.2014.00511, 2014. 


Fe-Oxide Grain Coatings Support Bacterial Fe-Reducing Metabolisms in 1.7-2.0 km-Deep Subsurface Quartz Arenite Sandstone Reservoirs of the Illinois Basin (USA), Y. Dong, R. A. Sanford, M. I. Boyanov, K. M. Kemner, T. M. Flynn, E. J. O’Loughlin, I. K. O. Cann, R. I. Mackie, R. A. Locke II, J. R. Weber, S. M. Egan, and B. W. Fouke, Frontiers in Microbiology, V. 5, Article 511, pp. 1-16. doi: 10.3389/fmicb.2014.00511, September 30, 2014. 



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